Bertil Hille #ionchannels and #GPCR and #KCNQ. #HilleTalk just starting! @UW

@IonChannelRtrt @millerlab Follow #HilleTalk for Bertil Hille talk of GPCRs, Ion Channels and more

@IonChannelRtrt @millerlab Follow #HilleTalk for Bertil Hille talk of GPCRs, Ion Channels and more

More than 40% of prescription drugs act on GPCR pathways #HilleTalk

I realize that my experiments are only in one cell and then I tell you about the whole brain, that is the way I see my results #HilleTalk

@UWMedicineNews follow #HilleTalk for Bertil Hille on GPCRs and Ion Channels

Neonatal seizures, deafness, long QT associated to potassium channel #HilleTalk

History of GPCR study. Cloning was what made thinks seem real. Sequencing put channel families together #HilleTalk

Broad phenomena, many Gq coupled receptors affecting ion channels #HilleTalk

How do Gq-mediated signals modulate ion channels? Focus on lipids! #HilleTalk

Manipulating lipids to affect ion channel currents: VSP 5-phosphatase has same effect on KCNQ currents as GPCR #HilleTalk

Using fluorescent markers to track phospholipid PIP2 on cell membrane. Oxo-M (GPCR) depletes PIP2 #HilleTalk

What about neurons without receptor over-expression? Oxo-M have real depletion of PIP2, enough to explain m-current modulation #HilleTalk

80 ion channels sensitive to PIP2 #HilleTalk

All Kv channels tested, only one sensitive to PIP2, in contrast all KCNQ are sensitive #HilleTalk

What about Cav channels? Beta subunits tune PIP2 sensitivity. b2a is membrane anchored, may provide channel anchoring #HilleTalk

Many other things sensitive to PIP2: Cytoskeleton, vesicle trafficking, not only ion channels #HilleTalk

Concept: phosphoinositides are localized to specific membranes of the cell. Specific enzymes for organelles change composition. #HilleTalk

Defect of phosphoinositides enzymes associated with lethal contractual syndrome, cataracts, glaucoma, liver fibrosis more. #HilleTalk

In conclusion GPCRs change your state of mind and membrane phosphoinositides regulate proteins! #HilleTalk

Thanks for following #HilleTalk, thanks to Bertil Hille for a great talk at @UW